《植物生理学报》 2017, 53(5): 772-780

甘薯‘徐薯18’转录组分析

颜朗, 魏昌赫, 张义正^*

四川大学生命科学学院, 四川省分子生物学及生物技术实验室, 成都610064

通信作者：张义正；E-mail: yizzhang@scu.edu.cn

摘　要：

‘徐薯18’是我国种植时间长和区域广的甘薯品种, 该品种的转录组数据库构建及其重要基因的挖掘, 对于甘薯遗传多样性分析和分子育种都具有重要意义。本文综述了我们近年来对‘徐薯18’转录组的研究进展, 包括利用第2代和第3代测序技术构建‘徐薯18’全长转录组数据库, 从转录组数据库中挖掘重要的功能基因以及分析它们的表达等, 并展望了未来甘薯转录组研究的发展方向。

关键词：甘薯; ‘徐薯18’; 转录组; 基因挖掘; 基因表达

收稿：2017-02-15   修定：2017-05-08

资助：国家科技支撑计划项目(2007BAD78B03)和四川省“十一五”重点攻关项目(07SG111-003-1)。

Transcriptomic analyses in sweetpotato [Ipomoea batatas (L.) Lam. cv. ‘Xushu 18’]

YAN Lang, WEI Chang-He, ZHANG Yi-Zheng^*

College of Life Sciences, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Sichuan University, Chengdu 610064, China

Corresponding author: ZHANG Yi-Zheng; E-mail: yizzhang@scu.edu.cn

Abstract:

The sweetpotato (Ipomoea batatas cv. ‘Xushu 18’) has a long period and wide area of cultivation in China. The construction of transcriptomic databases and the mining of functional genes of this cultivar will play significant roles in the genetic diversity analysis and molecular breeding of sweetpotato. This paper reviews the recent research progresses in ‘Xushu 18’ transcriptome, including the construction of full-length transcriptome databases using RNA-seq and Iso-seq, the mining of important functional genes and their expression. The future direction is discussed in the study of sweetpotato transcriptome.

Key words: sweetpotato; ‘Xushu 18’; transcriptome; gene mining; gene expression